|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD31 ORF mammalian expression plasmid, C-GFPSpark tag||RG80091-ACG|
|Rat CD31 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80091-ACR|
|Rat CD31 ORF mammalian expression plasmid, C-Flag tag||RG80091-CF|
|Rat CD31 ORF mammalian expression plasmid, C-His tag||RG80091-CH|
|Rat CD31 ORF mammalian expression plasmid, C-Myc tag||RG80091-CM|
|Rat CD31 ORF mammalian expression plasmid, C-HA tag||RG80091-CY|
|Rat CD31 Gene cDNA clone plasmid||RG80091-G|
|Rat CD31 ORF mammalian expression plasmid, N-Flag tag||RG80091-NF|
|Rat CD31 ORF mammalian expression plasmid, N-His tag||RG80091-NH|
|Rat CD31 ORF mammalian expression plasmid, N-Myc tag||RG80091-NM|
|Rat CD31 ORF mammalian expression plasmid, N-HA tag||RG80091-NY|
|Rat CD31 natural ORF mammalian expression plasmid||RG80091-UT|
|Learn more about expression Vectors|
The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.