|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat UCHL1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80089-ACG|
|Rat UCHL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80089-ACR|
|Rat UCHL1 ORF mammalian expression plasmid, N-GFPSpark tag||RG80089-ANG|
|Rat UCHL1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80089-ANR|
|Rat UCHL1 ORF mammalian expression plasmid, C-Flag tag||RG80089-CF|
|Rat UCHL1 ORF mammalian expression plasmid, C-His tag||RG80089-CH|
|Rat UCHL1 ORF mammalian expression plasmid, C-Myc tag||RG80089-CM|
|Rat UCHL1 ORF mammalian expression plasmid, C-HA tag||RG80089-CY|
|Rat UCHL1 Gene cDNA clone plasmid||RG80089-G|
|Rat UCHL1 ORF mammalian expression plasmid, N-Flag tag||RG80089-NF|
|Rat UCHL1 ORF mammalian expression plasmid, N-His tag||RG80089-NH|
|Rat UCHL1 ORF mammalian expression plasmid, N-Myc tag||RG80089-NM|
|Rat UCHL1 ORF mammalian expression plasmid, N-HA tag||RG80089-NY|
|Rat UCHL1 natural ORF mammalian expression plasmid||RG80089-UT|
|Learn more about expression Vectors|
Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.