|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat UCHL3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80087-ACG|
|Rat UCHL3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80087-ACR|
|Rat UCHL3 ORF mammalian expression plasmid, N-GFPSpark tag||RG80087-ANG|
|Rat UCHL3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80087-ANR|
|Rat UCHL3 ORF mammalian expression plasmid, C-Flag tag||RG80087-CF|
|Rat UCHL3 ORF mammalian expression plasmid, C-His tag||RG80087-CH|
|Rat UCHL3 ORF mammalian expression plasmid, C-Myc tag||RG80087-CM|
|Rat UCHL3 ORF mammalian expression plasmid, C-HA tag||RG80087-CY|
|Rat UCHL3 Gene cDNA clone plasmid||RG80087-G|
|Rat UCHL3 ORF mammalian expression plasmid, N-Flag tag||RG80087-NF|
|Rat UCHL3 ORF mammalian expression plasmid, N-His tag||RG80087-NH|
|Rat UCHL3 ORF mammalian expression plasmid, N-Myc tag||RG80087-NM|
|Rat UCHL3 ORF mammalian expression plasmid, N-HA tag||RG80087-NY|
|Rat UCHL3 natural ORF mammalian expression plasmid||RG80087-UT|
|Learn more about expression Vectors|
Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.