|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IL6 ORF mammalian expression plasmid, C-GFPSpark tag||RG80076-ACG|
|Rat IL6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80076-ACR|
|Rat IL6 ORF mammalian expression plasmid, C-Flag tag||RG80076-CF|
|Rat IL6 ORF mammalian expression plasmid, C-His tag||RG80076-CH|
|Rat IL6 ORF mammalian expression plasmid, C-Myc tag||RG80076-CM|
|Rat IL6 ORF mammalian expression plasmid, C-HA tag||RG80076-CY|
|Rat IL6 Gene cDNA clone plasmid||RG80076-M|
|Rat IL6 ORF mammalian expression plasmid, N-Flag tag||RG80076-NF|
|Rat IL6 ORF mammalian expression plasmid, N-His tag||RG80076-NH|
|Rat IL6 ORF mammalian expression plasmid, N-Myc tag||RG80076-NM|
|Rat IL6 ORF mammalian expression plasmid, N-HA tag||RG80076-NY|
|Rat IL6 natural ORF mammalian expression plasmid||RG80076-UT|
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Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.