|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IL18 ORF mammalian expression plasmid, C-GFPSpark tag||RG80072-ACG|
|Rat IL18 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80072-ACR|
|Rat IL18 ORF mammalian expression plasmid, C-Flag tag||RG80072-CF|
|Rat IL18 ORF mammalian expression plasmid, C-His tag||RG80072-CH|
|Rat IL18 ORF mammalian expression plasmid, C-Myc tag||RG80072-CM|
|Rat IL18 ORF mammalian expression plasmid, C-HA tag||RG80072-CY|
|Rat IL18 Gene cDNA clone plasmid||RG80072-M|
|Rat IL18 ORF mammalian expression plasmid, N-Flag tag||RG80072-NF|
|Rat IL18 ORF mammalian expression plasmid, N-His tag||RG80072-NH|
|Rat IL18 ORF mammalian expression plasmid, N-Myc tag||RG80072-NM|
|Rat IL18 ORF mammalian expression plasmid, N-HA tag||RG80072-NY|
|Rat IL18 natural ORF mammalian expression plasmid||RG80072-UT|
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Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.