|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat Neurexophilin-1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80047-ACG|
|Rat Neurexophilin-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80047-ACR|
|Rat Neurexophilin-1 ORF mammalian expression plasmid, C-Flag tag||RG80047-CF|
|Rat Neurexophilin-1 ORF mammalian expression plasmid, C-His tag||RG80047-CH|
|Rat Neurexophilin-1 ORF mammalian expression plasmid, C-Myc tag||RG80047-CM|
|Rat Neurexophilin-1 ORF mammalian expression plasmid, C-HA tag||RG80047-CY|
|Rat Neurexophilin-1 Gene cDNA clone plasmid||RG80047-M|
|Rat Neurexophilin-1 ORF mammalian expression plasmid, N-Flag tag||RG80047-NF|
|Rat Neurexophilin-1 ORF mammalian expression plasmid, N-His tag||RG80047-NH|
|Rat Neurexophilin-1 ORF mammalian expression plasmid, N-Myc tag||RG80047-NM|
|Rat Neurexophilin-1 ORF mammalian expression plasmid, N-HA tag||RG80047-NY|
|Rat Neurexophilin-1 natural ORF mammalian expression plasmid||RG80047-UT|
|Learn more about expression Vectors|
Neurexophilin-1, or NXPH1 is a secreted glycoprotein, which belongs to the Neurexophilin family. The Neurexophilin family contain at least four genes and resembles a neuropeptide, suggesting a function as an endogenous ligand for alpha-neurexins. The mammalian brains contain four genes for neurexophilins the products of which share a common structure composed of five domains: an N-terminal signal peptide, a variable N-terminal domain, a highly conserved central domain that is N-glycosylated, a short linker region, and a conserved C-terminal domain that is cysteine-rich. Neurexophilin-1 constitutes a secreted cysteine-rich glycoprotein, forms a very tight complex with alpha neurexins, a group of proteins that promote adhesion between dendrites and axons. Neurexophilins 1 and 3 but not 4 (neurexophilin 2 is not expressed in rodents) bind to a single individual LNS domain, the second overall LNS domain in all three alpha-neurexins.