|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD62L / SELL ORF mammalian expression plasmid, C-GFPSpark tag||RG80046-ACG|
|Rat CD62L / SELL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80046-ACR|
|Rat CD62L / SELL ORF mammalian expression plasmid, C-Flag tag||RG80046-CF|
|Rat CD62L / SELL ORF mammalian expression plasmid, C-His tag||RG80046-CH|
|Rat CD62L / SELL ORF mammalian expression plasmid, C-Myc tag||RG80046-CM|
|Rat CD62L / SELL ORF mammalian expression plasmid, C-HA tag||RG80046-CY|
|Rat CD62L / SELL Gene cDNA clone plasmid||RG80046-M|
|Rat CD62L / SELL ORF mammalian expression plasmid, N-Flag tag||RG80046-NF|
|Rat CD62L / SELL ORF mammalian expression plasmid, N-His tag||RG80046-NH|
|Rat CD62L / SELL ORF mammalian expression plasmid, N-Myc tag||RG80046-NM|
|Rat CD62L / SELL ORF mammalian expression plasmid, N-HA tag||RG80046-NY|
|Rat CD62L / SELL natural ORF mammalian expression plasmid||RG80046-UT|
|Learn more about expression Vectors|
L-selectin (SELL), also known as CD62L, is a key adhesion molecule that regulates both the migration of leukocytes at sites of inflammation and the recirculation of lymphocytes between blood and lymphoid tissues. It belongs to the selectin family of proteins, and consisting of a large, highly glycosylated, extracellular domain, a single spanning transmembrane domain and a small cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin-mediated lymphocyte recirculation is required for maintaining the appropriate tissue distribution of lymphocyte subpopulations including naïve and effector subsets such as regulatory T cells. In addition, L-selectin-mediated entry into peripheral lymph nodes is required for optimal induction of lymphocyte homeostatic proliferation during lymphopenia. Importantly, L-selectin has been shown to have both adhesive and signaling functions during leukocyte migration. L-selectin has also been shown to mediate leukocyte recruitment during chronic inflammatory and autoimmune diseases and thus is a potential therapeutic target for drug development.