|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat ACE2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80031-ACG|
|Rat ACE2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80031-ACR|
|Rat ACE2 ORF mammalian expression plasmid, C-Flag tag||RG80031-CF|
|Rat ACE2 ORF mammalian expression plasmid, C-His tag||RG80031-CH|
|Rat ACE2 ORF mammalian expression plasmid, C-Myc tag||RG80031-CM|
|Rat ACE2 ORF mammalian expression plasmid, C-HA tag||RG80031-CY|
|Rat ACE2 Gene cDNA clone plasmid||RG80031-M|
|Rat ACE2 ORF mammalian expression plasmid, N-Flag tag||RG80031-NF|
|Rat ACE2 ORF mammalian expression plasmid, N-His tag||RG80031-NH|
|Rat ACE2 ORF mammalian expression plasmid, N-Myc tag||RG80031-NM|
|Rat ACE2 ORF mammalian expression plasmid, N-HA tag||RG80031-NY|
|Rat ACE2 natural ORF mammalian expression plasmid||RG80031-UT|
|Learn more about expression Vectors|
Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme, an homolog of ACE, hydrolyzes angiotensin (Ang) I to produce Ang-(1-9), which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus, the major biologically active product of ACE2 is Ang-(1-7), which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension, cardiac function, heart function and diabetes, and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis.