|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD54 / ICAM1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80022-ACG|
|Rat CD54 / ICAM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80022-ACR|
|Rat CD54 / ICAM1 ORF mammalian expression plasmid, C-Flag tag||RG80022-CF|
|Rat CD54 / ICAM1 ORF mammalian expression plasmid, C-His tag||RG80022-CH|
|Rat CD54 / ICAM1 ORF mammalian expression plasmid, C-Myc tag||RG80022-CM|
|Rat CD54 / ICAM1 ORF mammalian expression plasmid, C-HA tag||RG80022-CY|
|Rat CD54 / ICAM1 Gene cDNA clone plasmid||RG80022-M|
|Rat CD54 / ICAM1 ORF mammalian expression plasmid, N-Flag tag||RG80022-NF|
|Rat CD54 / ICAM1 ORF mammalian expression plasmid, N-His tag||RG80022-NH|
|Rat CD54 / ICAM1 ORF mammalian expression plasmid, N-Myc tag||RG80022-NM|
|Rat CD54 / ICAM1 ORF mammalian expression plasmid, N-HA tag||RG80022-NY|
|Rat CD54 / ICAM1 natural ORF mammalian expression plasmid||RG80022-UT|
|Learn more about expression Vectors|
Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.