|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CPA1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50448-ACG|
|Mouse CPA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50448-ACR|
|Mouse CPA1 ORF mammalian expression plasmid, C-Flag tag||MG50448-CF|
|Mouse CPA1 ORF mammalian expression plasmid, C-His tag||MG50448-CH|
|Mouse CPA1 ORF mammalian expression plasmid, C-Myc tag||MG50448-CM|
|Mouse CPA1 ORF mammalian expression plasmid, C-HA tag||MG50448-CY|
|Mouse CPA1 Gene cDNA clone plasmid||MG50448-M|
|Mouse CPA1 ORF mammalian expression plasmid, N-Flag tag||MG50448-NF|
|Mouse CPA1 ORF mammalian expression plasmid, N-His tag||MG50448-NH|
|Mouse CPA1 ORF mammalian expression plasmid, N-Myc tag||MG50448-NM|
|Mouse CPA1 ORF mammalian expression plasmid, N-HA tag||MG50448-NY|
|Mouse CPA1 natural ORF mammalian expression plasmid||MG50448-UT|
|Learn more about expression Vectors|
Human Carboxypeptidase A1 （CPA1）is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.