|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse GPNMB ORF mammalian expression plasmid, C-GFPSpark tag||MG50475-ACG|
|Mouse GPNMB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50475-ACR|
|Mouse GPNMB ORF mammalian expression plasmid, C-Flag tag||MG50475-CF|
|Mouse GPNMB ORF mammalian expression plasmid, C-His tag||MG50475-CH|
|Mouse GPNMB ORF mammalian expression plasmid, C-Myc tag||MG50475-CM|
|Mouse GPNMB ORF mammalian expression plasmid, C-HA tag||MG50475-CY|
|Mouse GPNMB Gene cDNA clone plasmid||MG50475-M|
|Mouse GPNMB ORF mammalian expression plasmid, N-Flag tag||MG50475-NF|
|Mouse GPNMB ORF mammalian expression plasmid, N-His tag||MG50475-NH|
|Mouse GPNMB ORF mammalian expression plasmid, N-Myc tag||MG50475-NM|
|Mouse GPNMB ORF mammalian expression plasmid, N-HA tag||MG50475-NY|
|Mouse GPNMB natural ORF mammalian expression plasmid||MG50475-UT|
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GPNMB belongs to the PMEL / NMB family, also known as Osteoactivin and Hematopoietic growth factor-inducible neurokinin 1 ( HGFIN ), is a transmembrane glycoprotein that is expressed in numerous cells, including osteoclasts, macrophages, dendritic cells, and tumor cells. It is suggested to influence osteoblast maturation, cell adhesion and migration. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages. Osteoactivin is expressed at high levels in normal and inflammatory liver macrophages suggesting a significant role in acute liver injury. The early-phase upregulation of Osteoactivin expression in the tubular epithelium in response to renal injury might play a role in triggering renal interstitial fibrosis via activation of matrix metalloproteinase expression and collagen remodeling in rats. Osteoactivin as a protein that is expressed in aggressive human breast cancers and is capable of promoting breast cancer metastasis to bone.