|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse IFNAR1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50469-ACG|
|Mouse IFNAR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50469-ACR|
|Mouse IFNAR1 ORF mammalian expression plasmid, C-Flag tag||MG50469-CF|
|Mouse IFNAR1 ORF mammalian expression plasmid, C-His tag||MG50469-CH|
|Mouse IFNAR1 ORF mammalian expression plasmid, C-Myc tag||MG50469-CM|
|Mouse IFNAR1 ORF mammalian expression plasmid, C-HA tag||MG50469-CY|
|Mouse IFNAR1 Gene cDNA clone plasmid||MG50469-M|
|Mouse IFNAR1 ORF mammalian expression plasmid, N-Flag tag||MG50469-NF|
|Mouse IFNAR1 ORF mammalian expression plasmid, N-His tag||MG50469-NH|
|Mouse IFNAR1 ORF mammalian expression plasmid, N-Myc tag||MG50469-NM|
|Mouse IFNAR1 ORF mammalian expression plasmid, N-HA tag||MG50469-NY|
|Mouse IFNAR1 natural ORF mammalian expression plasmid||MG50469-UT|
|Learn more about expression Vectors|
Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.