|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse TIMP1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50342-ACG|
|Mouse TIMP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50342-ACR|
|Mouse TIMP1 ORF mammalian expression plasmid, C-Flag tag||MG50342-CF|
|Mouse TIMP1 ORF mammalian expression plasmid, C-His tag||MG50342-CH|
|Mouse TIMP1 ORF mammalian expression plasmid, C-Myc tag||MG50342-CM|
|Mouse TIMP1 ORF mammalian expression plasmid, C-HA tag||MG50342-CY|
|Mouse TIMP1 Gene cDNA clone plasmid||MG50342-M|
|Mouse TIMP1 ORF mammalian expression plasmid, N-Flag tag||MG50342-NF|
|Mouse TIMP1 ORF mammalian expression plasmid, N-His tag||MG50342-NH|
|Mouse TIMP1 ORF mammalian expression plasmid, N-Myc tag||MG50342-NM|
|Mouse TIMP1 ORF mammalian expression plasmid, N-HA tag||MG50342-NY|
|Mouse TIMP1 natural ORF mammalian expression plasmid||MG50342-UT|
|Learn more about expression Vectors|
TIMP metallopeptidase inhibitor 1, also known as TIMP-1/TIMP1, Collagenase inhibitor 16C8 fibroblast Erythroid-potentiating activity, TPA-S1TPA-induced proteinTissue inhibitor of metalloproteinases 1, is a natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. TIMP-1/TIMP1 is found in fetal and adult tissues. Highest levels are found in bone, lung, ovary and uterus. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. In addition to its inhibitory role against most of the known MMPs, the protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this protein encoding gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This encoding gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 is Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16.