|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse BTC ORF mammalian expression plasmid, C-GFPSpark tag||MG50340-ACG|
|Mouse BTC ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50340-ACR|
|Mouse BTC ORF mammalian expression plasmid, C-Flag tag||MG50340-CF|
|Mouse BTC ORF mammalian expression plasmid, C-His tag||MG50340-CH|
|Mouse BTC ORF mammalian expression plasmid, C-Myc tag||MG50340-CM|
|Mouse BTC ORF mammalian expression plasmid, C-HA tag||MG50340-CY|
|Mouse BTC Gene cDNA clone plasmid||MG50340-M|
|Mouse BTC ORF mammalian expression plasmid, N-Flag tag||MG50340-NF|
|Mouse BTC ORF mammalian expression plasmid, N-His tag||MG50340-NH|
|Mouse BTC ORF mammalian expression plasmid, N-Myc tag||MG50340-NM|
|Mouse BTC ORF mammalian expression plasmid, N-HA tag||MG50340-NY|
|Mouse BTC natural ORF mammalian expression plasmid||MG50340-UT|
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Betacellulin(BTC) is a member of the epidermal growth factor (EGF) family. These soluble proteins are ligands for one or more of the four receptor tyrosine kinases encoded by the ErbB gene family (ErbB-1/epidermal growth factor receptor (EGFR), neu/ErbB-2/HER2, ErbB-3/HER3 and ErbB-4/HER4). Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. This protein is a ligand for the EGF receptor. BTC is a polymer of about 62-111 amino acid residues. Secondary Structure: 6% helical (1 helices; 3 residues)36% beta sheet (5 strands; 18 residues). BTC was originally identified as a growth-promoting factor in mouse pancreatic β-cell carcinoma cell line and has since been identified in humans. It plays a role in the growth and development of the neonate and/or mammary gland function. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.