|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PREP ORF mammalian expression plasmid, C-GFPSpark tag||MG50288-ACG|
|Mouse PREP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50288-ACR|
|Mouse PREP ORF mammalian expression plasmid, N-GFPSpark tag||MG50288-ANG|
|Mouse PREP ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50288-ANR|
|Mouse PREP ORF mammalian expression plasmid, C-Flag tag||MG50288-CF|
|Mouse PREP ORF mammalian expression plasmid, C-His tag||MG50288-CH|
|Mouse PREP ORF mammalian expression plasmid, C-Myc tag||MG50288-CM|
|Mouse PREP ORF mammalian expression plasmid, C-HA tag||MG50288-CY|
|Mouse PREP Gene cDNA clone plasmid||MG50288-M|
|Mouse PREP ORF mammalian expression plasmid, N-Flag tag||MG50288-NF|
|Mouse PREP ORF mammalian expression plasmid, N-His tag||MG50288-NH|
|Mouse PREP ORF mammalian expression plasmid, N-Myc tag||MG50288-NM|
|Mouse PREP ORF mammalian expression plasmid, N-HA tag||MG50288-NY|
|Mouse PREP natural ORF mammalian expression plasmid||MG50288-UT|
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Prolyl endopeptidase, also known as PREP, belongs to a distinct class of serine peptidases. It is a large cytosolic enzyme which was first described in the cytosol of rabbit brain as an oligopeptidase. Prolyl endopeptidase degrades the nonapeptide bradykinin at the Pro-Phe bond. It is involved in the maturation and degradation of peptide hormones and neuropeptides such as alpha-melanocyte-stimulating hormone, luteinizing hormone-releasing hormone (LH-RH), thyrotropin-releasing hormone, angiotensin, neurotensin, oxytocin, substance P and vasopressin. Prolyl endopeptidase's activity is confined to action on oligopeptides of less than 10 kD and it has an absolute requirement for the trans-configuration of the peptide bond preceding proline. It cleaves peptide bonds at the C-terminal side of proline residues.