|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse TrkC ORF mammalian expression plasmid, C-GFPSpark tag||MG50320-ACG|
|Mouse TrkC ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50320-ACR|
|Mouse TrkC ORF mammalian expression plasmid, C-Flag tag||MG50320-CF|
|Mouse TrkC ORF mammalian expression plasmid, C-His tag||MG50320-CH|
|Mouse TrkC ORF mammalian expression plasmid, C-Myc tag||MG50320-CM|
|Mouse TrkC ORF mammalian expression plasmid, C-HA tag||MG50320-CY|
|Mouse TrkC Gene cDNA clone plasmid||MG50320-G|
|Mouse TrkC ORF mammalian expression plasmid, N-Flag tag||MG50320-NF|
|Mouse TrkC ORF mammalian expression plasmid, N-His tag||MG50320-NH|
|Mouse TrkC ORF mammalian expression plasmid, N-Myc tag||MG50320-NM|
|Mouse TrkC ORF mammalian expression plasmid, N-HA tag||MG50320-NY|
|Mouse TrkC natural ORF mammalian expression plasmid||MG50320-UT|
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NT-3 growth factor receptor also known as neurotrophic tyrosine kinase receptor type 3 or TrkC tyrosine kinase or Trk-C receptor, is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC/NTRK3 is widely expressed in the developing and adult nervous system. In later embryonic development, TrkC/NTRK3 is expressed in various structures of the CNS including the caudatoputamen, septal nuclei, cerebellum, and brainstem. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. In the PNS, trkC hybridization appears to correlate, both temporally and spatially, with the outgrowth of axons toward their peripheral targets. TrkC/NTRK3 is widely expressed in the three identified branches of the mammalian nervous system and appears to correlate with the expression of NT-3, its cognate ligand. The apparent colocalization of trkC transcripts with NT-3 raises the possibility this neurotrophin exerts its trophic effects by a paracrine and/or autocrine mechanism. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in TrkC encoding gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.