|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CD157 / BST1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50319-ACG|
|Mouse CD157 / BST1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50319-ACR|
|Mouse CD157 / BST1 ORF mammalian expression plasmid, C-Flag tag||MG50319-CF|
|Mouse CD157 / BST1 ORF mammalian expression plasmid, C-His tag||MG50319-CH|
|Mouse CD157 / BST1 ORF mammalian expression plasmid, C-Myc tag||MG50319-CM|
|Mouse CD157 / BST1 ORF mammalian expression plasmid, C-HA tag||MG50319-CY|
|Mouse CD157 / BST1 Gene cDNA clone plasmid||MG50319-M|
|Mouse CD157 / BST1 ORF mammalian expression plasmid, N-Flag tag||MG50319-NF|
|Mouse CD157 / BST1 ORF mammalian expression plasmid, N-His tag||MG50319-NH|
|Mouse CD157 / BST1 ORF mammalian expression plasmid, N-Myc tag||MG50319-NM|
|Mouse CD157 / BST1 ORF mammalian expression plasmid, N-HA tag||MG50319-NY|
|Mouse CD157 / BST1 natural ORF mammalian expression plasmid||MG50319-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD157, also known as ADP-ribosyl cyclase 2, is an ectoenzyme sharing several characteristics with ADP-ribosyl cyclase CD38. CD157 was originally identified as a bone marrow stromal cell molecule (BST-1) with a glycosylphosphatidylinositol (GPI) anchor to bind to the cell surface. CD157 is prevalently expressed by cells of the myeloid lineage. CD157 could act as a receptor with signal transduction capability. Further, it regulates calcium homeostasis and promotes polarization in neutrophils and mediates superoxide (O2−) production in the human U937 myeloid line.