|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ACP5 / TRAP ORF mammalian expression plasmid, C-GFPSpark tag||MG50274-ACG|
|Mouse ACP5 / TRAP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50274-ACR|
|Mouse ACP5 / TRAP ORF mammalian expression plasmid, C-Flag tag||MG50274-CF|
|Mouse ACP5 / TRAP ORF mammalian expression plasmid, C-His tag||MG50274-CH|
|Mouse ACP5 / TRAP ORF mammalian expression plasmid, C-Myc tag||MG50274-CM|
|Mouse ACP5 / TRAP ORF mammalian expression plasmid, C-HA tag||MG50274-CY|
|Mouse ACP5 / TRAP Gene cDNA clone plasmid||MG50274-M|
|Mouse ACP5 / TRAP ORF mammalian expression plasmid, N-Flag tag||MG50274-NF|
|Mouse ACP5 / TRAP ORF mammalian expression plasmid, N-His tag||MG50274-NH|
|Mouse ACP5 / TRAP ORF mammalian expression plasmid, N-Myc tag||MG50274-NM|
|Mouse ACP5 / TRAP ORF mammalian expression plasmid, N-HA tag||MG50274-NY|
|Mouse ACP5 / TRAP natural ORF mammalian expression plasmid||MG50274-UT|
|Learn more about expression Vectors|
Tartrate-resistant acid phosphatase (TRACP) or acid phosphatase 5, tartrate resistant (ACP5 or TRAP) is a glycosylated monomeric metalloenzyme expressed in mammals. TRACP is associated with osteoblast migration to bone resorption sites, and, once there, TRACP is believed to initiate osteoblast differentiation, activation, and proliferation. TRACP once considered to be just a histochemical marker of osteoclasts is now recognised to be a molecule of widespread occurrence with functions in both the skeleton and the immune system. Two forms of TRACP circulate in human blood, TRACP 5a derived from macrophages and dendritic cells, and TRACP-5b derived from osteoclasts. Recent data have demonstrated the utility of TRACP-5b as a marker of osteoclast number and bone resorption, and serum TRACP-5a as a marker of inflammatory conditions. TRACP is expressed by osteoclasts, macrophages, dendritic cells and a number of other cell types. It has a critical role in many biological processes including skeletal development, collagen synthesis and degradation, the mineralisation of bone, cytokine production by macrophages and dendritic cells, macrophage recruitment, dendritic cell maturation and a role in the development of Th1 responses.