|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ATF2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50262-ACG|
|Mouse ATF2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50262-ACR|
|Mouse ATF2 ORF mammalian expression plasmid, N-GFPSpark tag||MG50262-ANG|
|Mouse ATF2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50262-ANR|
|Mouse ATF2 ORF mammalian expression plasmid, C-Flag tag||MG50262-CF|
|Mouse ATF2 ORF mammalian expression plasmid, C-His tag||MG50262-CH|
|Mouse ATF2 ORF mammalian expression plasmid, C-Myc tag||MG50262-CM|
|Mouse ATF2 ORF mammalian expression plasmid, C-HA tag||MG50262-CY|
|Mouse ATF2 Gene cDNA clone plasmid||MG50262-M|
|Mouse ATF2 ORF mammalian expression plasmid, N-Flag tag||MG50262-NF|
|Mouse ATF2 ORF mammalian expression plasmid, N-His tag||MG50262-NH|
|Mouse ATF2 ORF mammalian expression plasmid, N-Myc tag||MG50262-NM|
|Mouse ATF2 ORF mammalian expression plasmid, N-HA tag||MG50262-NY|
|Mouse ATF2 natural ORF mammalian expression plasmid||MG50262-UT|
|Learn more about expression Vectors|
Activating transcription factor 2, also known as ATF2, is a member of the leucine zipper family of DNA-binding proteins that binds to the cAMP response element. Its activity is enhanced after phosphorylation by stress-activated protein kinases such as c-Jun N-terminal kinase and p38. ATF2 has been found to be a target of the JNK signal transduction pathway and mediate adenovirus E1A-inducible transcriptional activation. ATF2 is also been reported playing roles in TGF-β signaling pathway. It has been shown that the transcription factor ATF2 is bound by a hetero-oligomer of Smad3 and Smad4 upon TGF-β stimulation. Studies indicate that ATF-2 plays a central role in TGF-β signaling by acting as a common nuclear target of both Smad and TAK1 pathways.