|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse KLK-6 ORF mammalian expression plasmid, C-GFPSpark tag||MG50255-ACG|
|Mouse KLK-6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50255-ACR|
|Mouse KLK-6 ORF mammalian expression plasmid, C-Flag tag||MG50255-CF|
|Mouse KLK-6 ORF mammalian expression plasmid, C-His tag||MG50255-CH|
|Mouse KLK-6 ORF mammalian expression plasmid, C-Myc tag||MG50255-CM|
|Mouse KLK-6 ORF mammalian expression plasmid, C-HA tag||MG50255-CY|
|Mouse KLK-6 Gene cDNA clone plasmid||MG50255-M|
|Mouse KLK-6 ORF mammalian expression plasmid, N-Flag tag||MG50255-NF|
|Mouse KLK-6 ORF mammalian expression plasmid, N-His tag||MG50255-NH|
|Mouse KLK-6 ORF mammalian expression plasmid, N-Myc tag||MG50255-NM|
|Mouse KLK-6 ORF mammalian expression plasmid, N-HA tag||MG50255-NY|
|Mouse KLK-6 natural ORF mammalian expression plasmid||MG50255-UT|
|Learn more about expression Vectors|
KLK6 (kallikrein-related peptidase 6), also known as Klk7, belongs to the peptidase S1 family, Kallikrein subfamily. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. KLK6 is a serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Klk7 shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. KLK6 degrades alpha-synuclein and prevents its polymerization, indicating that KLK6 may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. Klk7 may be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.