|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse S100A10 ORF mammalian expression plasmid, C-GFPSpark tag||MG50252-ACG|
|Mouse S100A10 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50252-ACR|
|Mouse S100A10 ORF mammalian expression plasmid, N-GFPSpark tag||MG50252-ANG|
|Mouse S100A10 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50252-ANR|
|Mouse S100A10 ORF mammalian expression plasmid, C-Flag tag||MG50252-CF|
|Mouse S100A10 ORF mammalian expression plasmid, C-His tag||MG50252-CH|
|Mouse S100A10 ORF mammalian expression plasmid, C-Myc tag||MG50252-CM|
|Mouse S100A10 ORF mammalian expression plasmid, C-HA tag||MG50252-CY|
|小鼠 S100A10 基因全长ORF克隆||MG50252-M|
|Mouse S100A10 ORF mammalian expression plasmid, N-Flag tag||MG50252-NF|
|Mouse S100A10 ORF mammalian expression plasmid, N-His tag||MG50252-NH|
|Mouse S100A10 ORF mammalian expression plasmid, N-Myc tag||MG50252-NM|
|Mouse S100A10 ORF mammalian expression plasmid, N-HA tag||MG50252-NY|
|Mouse S100A10 natural ORF mammalian expression plasmid||MG50252-UT|
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S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.
Protein S100-A10, also known as Calpactin I light chain, Cellular ligand of annexin II, S100 calcium-binding protein A10, p10 protein, p11, ANX2LG and S100A10, is a member of the S100 family of small, dimeric EF hand-type Ca(2+)-binding proteins that generally modulate cellular target proteins in response to intracellular Ca(2+) signals. In contrast to all other S100 proteins, S100A10 is Ca(2+) insensitive because of amino acid replacements in its Ca(2+)-binding loops that lock the protein in a permanently active state. S100A10 forms a heterotetramer with annexin IIH and promotes carcinoma invasion and metastasis by plasminogen activation. S100A10 and annexin II contribute to the aggressive characteristics of anaplastic carcinoma, while playing a constitutive role in papillary carcinoma. S100A10 induces the dimerization of ANXA2 / p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target of tyrosine-specific kinase. S100A10 functions as a linker tethering certain transmembrane proteins to annexin A2 thereby assisting their traffic to the plasma membrane and/or their firm anchorage at certain membrane sites.