|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PPIA ORF mammalian expression plasmid, C-GFPSpark tag||MG50296-ACG|
|Mouse PPIA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50296-ACR|
|Mouse PPIA ORF mammalian expression plasmid, C-Flag tag||MG50296-CF|
|Mouse PPIA ORF mammalian expression plasmid, C-His tag||MG50296-CH|
|Mouse PPIA ORF mammalian expression plasmid, C-Myc tag||MG50296-CM|
|Mouse PPIA ORF mammalian expression plasmid, C-HA tag||MG50296-CY|
|Mouse PPIA Gene cDNA clone plasmid||MG50296-M|
|Mouse PPIA ORF mammalian expression plasmid, N-Flag tag||MG50296-NF|
|Mouse PPIA ORF mammalian expression plasmid, N-His tag||MG50296-NH|
|Mouse PPIA ORF mammalian expression plasmid, N-Myc tag||MG50296-NM|
|Mouse PPIA ORF mammalian expression plasmid, N-HA tag||MG50296-NY|
|Mouse PPIA natural ORF mammalian expression plasmid||MG50296-UT|
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Mouse peptidyl-prolyl cis-trans isomerase A, also known as PPIase A, Rotamase A, Cyclophilin A, Cyclosporin A-binding protein, PPIA and CYPA, is a cytoplasm protein which belongs to the cyclophilin-type PPIase family and PPIase A subfamily. Cyclophilins (CyPs) are a family of proteins found in organisms ranging from prokaryotes to humans. These molecules exhibit peptidyl-prolyl isomerase activity, suggesting that they influence the conformation of proteins in cells. PPIA / Cyclophilin A accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA / Cyclophilin A is secreted by vascular smooth muscle cells in response to inflammatory stimuli, and could thus contribute to atherosclerosis. It is not essential for mammalian cell viability. PPIA / Cyclophilin A can interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions.