|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse C1QB ORF mammalian expression plasmid, C-GFPSpark tag||MG50295-ACG|
|Mouse C1QB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50295-ACR|
|Mouse C1QB ORF mammalian expression plasmid, C-Flag tag||MG50295-CF|
|Mouse C1QB ORF mammalian expression plasmid, C-His tag||MG50295-CH|
|Mouse C1QB ORF mammalian expression plasmid, C-Myc tag||MG50295-CM|
|Mouse C1QB ORF mammalian expression plasmid, C-HA tag||MG50295-CY|
|Mouse C1QB Gene cDNA clone plasmid||MG50295-M|
|Mouse C1QB ORF mammalian expression plasmid, N-Flag tag||MG50295-NF|
|Mouse C1QB ORF mammalian expression plasmid, N-His tag||MG50295-NH|
|Mouse C1QB ORF mammalian expression plasmid, N-Myc tag||MG50295-NM|
|Mouse C1QB ORF mammalian expression plasmid, N-HA tag||MG50295-NY|
|Mouse C1QB natural ORF mammalian expression plasmid||MG50295-UT|
|Learn more about expression Vectors|
Complement Component 1, q subcomponent (C1q) associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Southern blot analysis of chromosomal DNA from vertebrate species demonstrated highest similarity between the C1qB genes, followed by C1qC and finally C1qA. Sequence comparison of C1q from three different species have shown that the B chains have the strongest similarity. C1q was already present at embryonic day 14 (E14) and showed little change in abundance through six weeks postnatal. At E16, C1qB mRNA was present at high abundance in putative microglia/macrophages in cortical marginal and intermediate zones, and hippocampal analge.