|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human KLRF1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10984-ACG|
|Human KLRF1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10984-ACR|
|Human KLRF1 ORF mammalian expression plasmid, C-Flag tag||HG10984-CF|
|Human KLRF1 ORF mammalian expression plasmid, C-His tag||HG10984-CH|
|Human KLRF1 ORF mammalian expression plasmid, C-Myc tag||HG10984-CM|
|Human KLRF1 ORF mammalian expression plasmid, C-HA tag||HG10984-CY|
|Human KLRF1 Gene cDNA clone plasmid||HG10984-M|
|Human KLRF1 ORF mammalian expression plasmid, N-Flag tag||HG10984-NF|
|Human KLRF1 ORF mammalian expression plasmid, N-His tag||HG10984-NH|
|Human KLRF1 ORF mammalian expression plasmid, N-Myc tag||HG10984-NM|
|Human KLRF1 ORF mammalian expression plasmid, N-HA tag||HG10984-NY|
|Human KLRF1 natural ORF mammalian expression plasmid||HG10984-UT|
|Learn more about expression Vectors|
NKp80, also known as KLRF1, is an activating homodimeric C-type lectin-like receptor which is expressed on nearly all natural killer cells and stimulates their cytoxicity and cytokine release. NKp80 stimulates cytotoxicity upon engagement of its genetically linked ligand: myeloid-specific CTLR activation-induced C-type lectin (AICL). NKp80, but not NKp80 mutated at tyrosine 7 (NKp80/Y7F), is tyrosine phosphorylated. Accordingly, NKp80/Y7F, but not NKp80/Y30F or NKp80/Y37F, failed to induce cytotoxicity. NKp80 phosphopeptides comprising the hemi-ITAM-like sequence surrounding tyrosine 7 bound Lck- and Syk-family kinases; accordingly, cross-linking of NKp80, but not NKp80/Y7F, induced Syk phosphorylation. Moreover, inhibition of Syk kinase, but not ZAP-70 kinase, impaired cytotoxic responses through NKp80. Atypical residues in the hemi-ITAM-like motif of NKp80 cause an altered stoichiometry of phosphorylation but did not substantially affect NK cytotoxicity. Altogether, these results show that NKp80 uses an atypical hemi-ITAM and Syk kinase to trigger cellular cytotoxicity.