|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human CD8A ORF mammalian expression plasmid, C-GFPSpark tag||HG10980-ACG|
|Human CD8A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10980-ACR|
|Human CD8A ORF mammalian expression plasmid, C-Flag tag||HG10980-CF|
|Human CD8A ORF mammalian expression plasmid, C-His tag||HG10980-CH|
|Human CD8A ORF mammalian expression plasmid, C-Myc tag||HG10980-CM|
|Human CD8A ORF mammalian expression plasmid, C-HA tag||HG10980-CY|
|Human CD8A Gene cDNA clone plasmid||HG10980-M|
|Human CD8A ORF mammalian expression plasmid, N-Flag tag||HG10980-NF|
|Human CD8A ORF mammalian expression plasmid, N-His tag||HG10980-NH|
|Human CD8A ORF mammalian expression plasmid, N-Myc tag||HG10980-NM|
|Human CD8A ORF mammalian expression plasmid, N-HA tag||HG10980-NY|
|Human CD8A natural ORF mammalian expression plasmid||HG10980-UT|
|Learn more about expression Vectors|
Human T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.