|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse MARCO ORF mammalian expression plasmid, C-GFPSpark tag||MG50283-ACG|
|Mouse MARCO ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50283-ACR|
|Mouse MARCO ORF mammalian expression plasmid, C-Flag tag||MG50283-CF|
|Mouse MARCO ORF mammalian expression plasmid, C-His tag||MG50283-CH|
|Mouse MARCO ORF mammalian expression plasmid, C-Myc tag||MG50283-CM|
|Mouse MARCO ORF mammalian expression plasmid, C-HA tag||MG50283-CY|
|Mouse MARCO Gene cDNA clone plasmid||MG50283-M|
|Mouse MARCO ORF mammalian expression plasmid, N-Flag tag||MG50283-NF|
|Mouse MARCO ORF mammalian expression plasmid, N-His tag||MG50283-NH|
|Mouse MARCO ORF mammalian expression plasmid, N-Myc tag||MG50283-NM|
|Mouse MARCO ORF mammalian expression plasmid, N-HA tag||MG50283-NY|
|Mouse MARCO natural ORF mammalian expression plasmid||MG50283-UT|
|Learn more about expression Vectors|
Macrophage receptor MARCO, also known as Macrophage receptor with collagenous structure and Marco, is a single-pass type I I membrane protein. MARCO is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. It is expressed in subpopulations of macrophages in the spleen and the medullary cord of lymph nodes. Although it is expressed on subsets of macrophages, it can be upregulated on other macrophages after bacterial infection. The strategic position of MARCO-expressing cells in lymphoid organs suggests an important role for this bacteria-binding molecule in removal of pathogens. MARCO has a short N-terminal cytoplasmic domain, a transmembrane domain, and a large extracellular part composed of a 75-residue long spacer domain, a 270-residue collagenous domain, and a 99-residue long scavenger receptor cysteine-rich (SRCR) domain. It is possible that cooperation between the SRCR domain and the collagenous domain is needed for high-affinity bacterial binding, or that the SRCR domain has to be in a trimeric form to effectively bind to bacteria