|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human IL10 ORF mammalian expression plasmid, C-GFPSpark tag||HG10947-ACG|
|Human IL10 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10947-ACR|
|Human IL10 ORF mammalian expression plasmid, C-Flag tag||HG10947-CF|
|Human IL10 ORF mammalian expression plasmid, C-His tag||HG10947-CH|
|Human IL10 ORF mammalian expression plasmid, C-Myc tag||HG10947-CM|
|Human IL10 ORF mammalian expression plasmid, C-HA tag||HG10947-CY|
|Human IL10 Gene cDNA clone plasmid||HG10947-M|
|Human IL10 natural ORF mammalian expression plasmid||HG10947-M-N|
|Human IL10 ORF mammalian expression plasmid, N-Flag tag||HG10947-NF|
|Human IL10 ORF mammalian expression plasmid, N-His tag||HG10947-NH|
|Human IL10 ORF mammalian expression plasmid, N-Myc tag||HG10947-NM|
|Human IL10 ORF mammalian expression plasmid, N-HA tag||HG10947-NY|
|Human IL10 natural ORF mammalian expression plasmid||HG10947-UT|
|Learn more about expression Vectors|
IL-10 is a anti-inflammatory cytokine which belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.