|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SDF2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50241-ACG|
|Mouse SDF2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50241-ACR|
|Mouse SDF2 ORF mammalian expression plasmid, C-Flag tag||MG50241-CF|
|Mouse SDF2 ORF mammalian expression plasmid, C-His tag||MG50241-CH|
|Mouse SDF2 ORF mammalian expression plasmid, C-Myc tag||MG50241-CM|
|Mouse SDF2 ORF mammalian expression plasmid, C-HA tag||MG50241-CY|
|Mouse SDF2 Gene cDNA clone plasmid||MG50241-M|
|Mouse SDF2 ORF mammalian expression plasmid, N-Flag tag||MG50241-NF|
|Mouse SDF2 ORF mammalian expression plasmid, N-His tag||MG50241-NH|
|Mouse SDF2 ORF mammalian expression plasmid, N-Myc tag||MG50241-NM|
|Mouse SDF2 ORF mammalian expression plasmid, N-HA tag||MG50241-NY|
|Mouse SDF2 natural ORF mammalian expression plasmid||MG50241-UT|
|Learn more about expression Vectors|
Stromal derived factors (SDFs) are a loosely defined group of molecules that are generated by stromal cells. Two of the stromal derived factors, SDF-1 and SDF-4 belong to the chemokine family. Other SDFs, such as SDF-2 and SDF-5 are not well defined and their biological functions are less known. SDF-2 is first isolated from the mouse stromal cell line ST2 as a secretory protein. The amino acid sequence deduced from the murine clone and the human homolog are conserved more than 92 %, and the aa sequence of SDF-2 shows similarity to those of yeast dolichyl phosphate-D-mannose, protein mannosyltransferases. SDF-1 and its receptor are strongly indicated in the progression of various cancers including breast cancer. SDF-2, SDF2-L1, SDF-4, and SDF-5 are ubiquitously expressed in various cancer cell lines and SDF-2, SDF-4 and SDF-5 are expressed in mammary tissues. These SDFs have prognostic value and warrant further investigation in their biological functions and clinical value.