|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse C1QBP ORF mammalian expression plasmid, C-GFPSpark tag||MG50199-ACG|
|Mouse C1QBP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50199-ACR|
|Mouse C1QBP ORF mammalian expression plasmid, N-GFPSpark tag||MG50199-ANG|
|Mouse C1QBP ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50199-ANR|
|Mouse C1QBP ORF mammalian expression plasmid, C-Flag tag||MG50199-CF|
|Mouse C1QBP ORF mammalian expression plasmid, C-His tag||MG50199-CH|
|Mouse C1QBP ORF mammalian expression plasmid, C-Myc tag||MG50199-CM|
|Mouse C1QBP ORF mammalian expression plasmid, C-HA tag||MG50199-CY|
|Mouse C1QBP Gene cDNA clone plasmid||MG50199-M|
|Mouse C1QBP ORF mammalian expression plasmid, N-Flag tag||MG50199-NF|
|Mouse C1QBP ORF mammalian expression plasmid, N-His tag||MG50199-NH|
|Mouse C1QBP ORF mammalian expression plasmid, N-Myc tag||MG50199-NM|
|Mouse C1QBP ORF mammalian expression plasmid, N-HA tag||MG50199-NY|
|Mouse C1QBP natural ORF mammalian expression plasmid||MG50199-UT|
|Learn more about expression Vectors|
Hyaluronan binding protein 1 (HABP1), also known as p32 or gC1qR, is a ubiquitously expressed multifunctional phospho-protein implicated in cell signalling. Hyaluronan-binding protein 1 (HABP1) /p32/gC1qR was characterized as a highly acidic and oligomeric protein, which binds to different ligands like hyaluronan, C1q, and mannosylated albumin. The role of hyaluronan binding protein 1 (HABP1) in cell signaling was investigated and in vitro. HABP1 overexpressing cells showed extensive vacuolation and reduced growth rate, which was corrected by frequent medium replenishment. Further investigation revealed that HABP1 overexpressing cells undergo apoptosis, and they failed to enter into the S-phase. The sperm surface HABP1 level can be correlated with the degree of sperm motility.Hyaluronan binding protein 1 (HABP1) was reported to be present on human sperm surface and its involvement in fertilization has already been elucidated: decreased HABP1 level may be associated with low motility of sperms, which in turn might cause infertility in the patient. HABP1 also is an endogenous substrate for MAP kinase and upon mitogenic stimulation it is translocated to the nucleus in a MAP kinase-dependent manner.