|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse VSIG4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50187-ACG|
|Mouse VSIG4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50187-ACR|
|Mouse VSIG4 ORF mammalian expression plasmid, C-Flag tag||MG50187-CF|
|Mouse VSIG4 ORF mammalian expression plasmid, C-His tag||MG50187-CH|
|Mouse VSIG4 ORF mammalian expression plasmid, C-Myc tag||MG50187-CM|
|Mouse VSIG4 ORF mammalian expression plasmid, C-HA tag||MG50187-CY|
|Mouse VSIG4 Gene cDNA clone plasmid||MG50187-M|
|Mouse VSIG4 ORF mammalian expression plasmid, N-Flag tag||MG50187-NF|
|Mouse VSIG4 ORF mammalian expression plasmid, N-His tag||MG50187-NH|
|Mouse VSIG4 ORF mammalian expression plasmid, N-Myc tag||MG50187-NM|
|Mouse VSIG4 ORF mammalian expression plasmid, N-HA tag||MG50187-NY|
|Mouse VSIG4 natural ORF mammalian expression plasmid||MG50187-UT|
|Learn more about expression Vectors|
VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type I g domain and a truncated C-type I g domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 280 amino acid precursor that contains a signal sequence, an V-type I g domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type I g domain of mouse VSIG4 shares 86% and 80% aa sequence identity with the V-type I g domains of rat and human VSIG4, respectively.