|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse S100A7A ORF mammalian expression plasmid, C-GFPSpark tag||MG50226-ACG|
|Mouse S100A7A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50226-ACR|
|Mouse S100A7A ORF mammalian expression plasmid, N-GFPSpark tag||MG50226-ANG|
|Mouse S100A7A ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50226-ANR|
|Mouse S100A7A ORF mammalian expression plasmid, C-Flag tag||MG50226-CF|
|Mouse S100A7A ORF mammalian expression plasmid, C-His tag||MG50226-CH|
|Mouse S100A7A ORF mammalian expression plasmid, C-Myc tag||MG50226-CM|
|Mouse S100A7A ORF mammalian expression plasmid, C-HA tag||MG50226-CY|
|Mouse S100A7A Gene cDNA clone plasmid||MG50226-M|
|Mouse S100A7A ORF mammalian expression plasmid, N-Flag tag||MG50226-NF|
|Mouse S100A7A ORF mammalian expression plasmid, N-His tag||MG50226-NH|
|Mouse S100A7A ORF mammalian expression plasmid, N-Myc tag||MG50226-NM|
|Mouse S100A7A ORF mammalian expression plasmid, N-HA tag||MG50226-NY|
|Mouse S100A7A natural ORF mammalian expression plasmid||MG50226-UT|
|Learn more about expression Vectors|
Koebnerisin is also known as protein S100-A7A (S100A7A), S100 calcium-binding protein A7-like 1 (S100A7L1) or S100 calcium-binding protein A15 (S100A15). Human S100A7A / S100A15 is a novel member of the S100 family of EF-hand calcium-binding proteins and was recently identified in psoriasis, where it is significantly upregulated in lesional skin. S100A7 is expressed by both normal cultured and malignant keratinocytes and malignant breast epithelial cells within ductal carcinoma in situ, suggesting an association with abnormal pathways of differentiation. S100A7 plays a role in the pathogenesis of inflammatory skin disease, as a chemotactic factor for hematopoietic cells. It also plays a role in early stages of breast tumor progression in association with the development of the invasive phenotype. The association of the 11.2 kDa S100A7A / S100A15 with psoriasis suggests that it contributes to the pathogenesis of the disease and could provide a molecular target for therapy.