|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse IL36RN / IL1F5 ORF mammalian expression plasmid, C-GFPSpark tag||MG50213-ACG|
|Mouse IL36RN / IL1F5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50213-ACR|
|Mouse IL36RN / IL1F5 ORF mammalian expression plasmid, C-Flag tag||MG50213-CF|
|Mouse IL36RN / IL1F5 ORF mammalian expression plasmid, C-His tag||MG50213-CH|
|Mouse IL36RN / IL1F5 ORF mammalian expression plasmid, C-Myc tag||MG50213-CM|
|Mouse IL36RN / IL1F5 ORF mammalian expression plasmid, C-HA tag||MG50213-CY|
|Mouse IL36RN / IL1F5 Gene cDNA clone plasmid||MG50213-M|
|Mouse IL36RN / IL1F5 ORF mammalian expression plasmid, N-Flag tag||MG50213-NF|
|Mouse IL36RN / IL1F5 ORF mammalian expression plasmid, N-His tag||MG50213-NH|
|Mouse IL36RN / IL1F5 ORF mammalian expression plasmid, N-Myc tag||MG50213-NM|
|Mouse IL36RN / IL1F5 ORF mammalian expression plasmid, N-HA tag||MG50213-NY|
|Mouse IL36RN / IL1F5 natural ORF mammalian expression plasmid||MG50213-UT|
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Interleukin-1 family member 5 (IL-1F5), also known as interleukin 36 receptor antagonist (IL36RA), is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). IL-1F5 could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), which is present in epithelial barriers and takes part in local inflammatory response. It has been proved that IL-1F5 induces IL-4 mRNA and protein expression in glia in vitro and enhances hippocampal expression of IL-4 following intracerebroventricular injection. The inhibitory effect of IL-1F5 on LPS-induced IL-1β is attenuated in cells from IL-4-defective mice. Experiment results suggest that IL-1F5 mediates anti-inflammatory effects through its ability to induce IL-4 production and that this is a consequence of its interaction with the orphan receptor, single Ig IL-1R-related molecule (SIGIRR)/TIR8, as the effects were not observed in SIGIRR−/− mice. In contrast to its effects in brain tissue, IL-1F5 did not attenuate LPS-induced changes, or up-regulated IL-4 in macrophages or dendritic cells, suggesting that the effect is confined to the brain.