|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PLAUR ORF mammalian expression plasmid, C-GFPSpark tag||MG50160-ACG|
|Mouse PLAUR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50160-ACR|
|Mouse PLAUR ORF mammalian expression plasmid, C-Flag tag||MG50160-CF|
|Mouse PLAUR ORF mammalian expression plasmid, C-His tag||MG50160-CH|
|Mouse PLAUR ORF mammalian expression plasmid, C-Myc tag||MG50160-CM|
|Mouse PLAUR ORF mammalian expression plasmid, C-HA tag||MG50160-CY|
|Mouse PLAUR Gene cDNA clone plasmid||MG50160-M|
|Mouse PLAUR ORF mammalian expression plasmid, N-Flag tag||MG50160-NF|
|Mouse PLAUR ORF mammalian expression plasmid, N-His tag||MG50160-NH|
|Mouse PLAUR ORF mammalian expression plasmid, N-Myc tag||MG50160-NM|
|Mouse PLAUR ORF mammalian expression plasmid, N-HA tag||MG50160-NY|
|Mouse PLAUR natural ORF mammalian expression plasmid||MG50160-UT|
|Learn more about expression Vectors|
Urokinase plasminogen activator (uPA) and/or its receptor (uPAR) are essential for metastasis, and overexpression of these molecules is strongly correlated with poor prognosis in a variety of malignant tumours. uPAR and uPA levels in both resected tumor tissue and plasma are of independent prognostic significance for patient survival in several types of human cancer. This system has classically been thought to drive tumor progression by mediating directed extracellular proteolysis on the surface of migrating or invading cells, and intervening with this proteolysis by targeting uPAR has been proposed to represent a novel approach for inhibiting tumor progression. uPAR, also known as PLAUR or CD87, has been implicated in the growth, metastasis, and angiogenesis of several solid and hemotologic malignancies. uPAR is a highly glycosylated, 55-60kDa integral membrane protein linked to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. It is part of a cell surface system that also consists of the serine protease uPA and several specific inhibitors (plasminogen activator inhibitors 1 and 2). Additionally, the analysis of CD87 (urokinase-type plasminogen activator receptor - uPAR) expression has a potential role in the diagnostic or prognostic work-up of several hematological malignancies, particularly acute leukemia and multiple myeloma.