|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse FGFR4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50194-ACG|
|Mouse FGFR4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50194-ACR|
|Mouse FGFR4 ORF mammalian expression plasmid, C-Flag tag||MG50194-CF|
|Mouse FGFR4 ORF mammalian expression plasmid, C-His tag||MG50194-CH|
|Mouse FGFR4 ORF mammalian expression plasmid, C-Myc tag||MG50194-CM|
|Mouse FGFR4 ORF mammalian expression plasmid, C-HA tag||MG50194-CY|
|Mouse FGFR4 Gene cDNA clone plasmid||MG50194-M|
|Mouse FGFR4 ORF mammalian expression plasmid, N-Flag tag||MG50194-NF|
|Mouse FGFR4 ORF mammalian expression plasmid, N-His tag||MG50194-NH|
|Mouse FGFR4 ORF mammalian expression plasmid, N-Myc tag||MG50194-NM|
|Mouse FGFR4 ORF mammalian expression plasmid, N-HA tag||MG50194-NY|
|Mouse FGFR4 natural ORF mammalian expression plasmid||MG50194-UT|
|Learn more about expression Vectors|
Fibroblast growth factor receptor 4 (FGFR4) also known as CD334 antigen or tyrosine kinase related to fibroblast growth factor receptor, is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR4/CD334 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR4/CD334 preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. FGFR4/CD334 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4/CD334. Mutations in FGFR4/CD334 lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.