|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse TREM2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50149-ACG|
|Mouse TREM2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50149-ACR|
|Mouse TREM2 ORF mammalian expression plasmid, C-Flag tag||MG50149-CF|
|Mouse TREM2 ORF mammalian expression plasmid, C-His tag||MG50149-CH|
|Mouse TREM2 ORF mammalian expression plasmid, C-Myc tag||MG50149-CM|
|Mouse TREM2 ORF mammalian expression plasmid, C-HA tag||MG50149-CY|
|Mouse TREM2 Gene cDNA clone plasmid||MG50149-M|
|Mouse TREM2 ORF mammalian expression plasmid, N-Flag tag||MG50149-NF|
|Mouse TREM2 ORF mammalian expression plasmid, N-His tag||MG50149-NH|
|Mouse TREM2 ORF mammalian expression plasmid, N-Myc tag||MG50149-NM|
|Mouse TREM2 ORF mammalian expression plasmid, N-HA tag||MG50149-NY|
|Mouse TREM2 natural ORF mammalian expression plasmid||MG50149-UT|
|Learn more about expression Vectors|
Triggering receptor expressed on myeloid cells 2 ( TREM2 ) is a single Ig domain receptor. It is expressed on macrophages and dendritic cells but not on granulocytes or monocytes. Its expression is most abundant in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, and microglial cells are the major TREM2-producing cell type in the central nervous system (CNS). TREM2 may play a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. It also associates with the signal adapter protein, DAP12, which has a cytoplasmic ITAM, leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is both required and sufficient for competent uptake of apoptotic neuronal cells. TREM2 and TREM2-L form a receptor-ligand pair connecting microglia with apoptotic neurons, directing removal of damaged cells to allow repair. Deficiency of the adapter protein DAP12 or its associated receptor TREM2 is associated with abnormal osteoclast development in humans. Defects in TREM2 are causes of PLOSL, also known as NHD. In addition, TREM2 signaling is also an important pathway to promote healing of wounds in the colon where stem cell replacement is necessary.