|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CXCL4 / PF4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50144-ACG|
|Mouse CXCL4 / PF4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50144-ACR|
|Mouse CXCL4 / PF4 ORF mammalian expression plasmid, C-Flag tag||MG50144-CF|
|Mouse CXCL4 / PF4 ORF mammalian expression plasmid, C-His tag||MG50144-CH|
|Mouse CXCL4 / PF4 ORF mammalian expression plasmid, C-Myc tag||MG50144-CM|
|Mouse CXCL4 / PF4 ORF mammalian expression plasmid, C-HA tag||MG50144-CY|
|Mouse CXCL4 / PF4 Gene cDNA clone plasmid||MG50144-M|
|Mouse CXCL4 / PF4 ORF mammalian expression plasmid, N-Flag tag||MG50144-NF|
|Mouse CXCL4 / PF4 ORF mammalian expression plasmid, N-His tag||MG50144-NH|
|Mouse CXCL4 / PF4 ORF mammalian expression plasmid, N-Myc tag||MG50144-NM|
|Mouse CXCL4 / PF4 ORF mammalian expression plasmid, N-HA tag||MG50144-NY|
|Mouse CXCL4 / PF4 natural ORF mammalian expression plasmid||MG50144-UT|
|Learn more about expression Vectors|
Platelet factor 4 (PF4), also known as chemokine (C-X-C motif) ligand 4 (CXCL4), is a small cytokine belonging to the CXC chemokine family. CXCL4/PF4 is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, CXCL4/PF4 probably has a role in inflammation and wound repair. This protein is released during platelet aggregation. CXCL4/PF4 neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 is chemotactic for neutrophils and monocytes. It inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. CXCL4/PF4 is up-regulated in human liver fibrosis and that it plays a nonredundant, functional role in experimental liver fibrosis by mediating stellate cell proliferation, migration, and intrahepatic immune cell recruitment.