|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse TNFSF12 ORF mammalian expression plasmid, C-GFPSpark tag||MG50174-ACG|
|Mouse TNFSF12 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50174-ACR|
|Mouse TNFSF12 ORF mammalian expression plasmid, C-Flag tag||MG50174-CF|
|Mouse TNFSF12 ORF mammalian expression plasmid, C-His tag||MG50174-CH|
|Mouse TNFSF12 ORF mammalian expression plasmid, C-Myc tag||MG50174-CM|
|Mouse TNFSF12 ORF mammalian expression plasmid, C-HA tag||MG50174-CY|
|Mouse TNFSF12 Gene cDNA clone plasmid||MG50174-M|
|Mouse TNFSF12 ORF mammalian expression plasmid, N-Flag tag||MG50174-NF|
|Mouse TNFSF12 ORF mammalian expression plasmid, N-His tag||MG50174-NH|
|Mouse TNFSF12 ORF mammalian expression plasmid, N-Myc tag||MG50174-NM|
|Mouse TNFSF12 ORF mammalian expression plasmid, N-HA tag||MG50174-NY|
|Mouse TNFSF12 natural ORF mammalian expression plasmid||MG50174-UT|
|Learn more about expression Vectors|
TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.