|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ACVR2B ORF mammalian expression plasmid, C-GFPSpark tag||MG50173-ACG|
|Mouse ACVR2B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50173-ACR|
|Mouse ACVR2B ORF mammalian expression plasmid, C-Flag tag||MG50173-CF|
|Mouse ACVR2B ORF mammalian expression plasmid, C-His tag||MG50173-CH|
|Mouse ACVR2B ORF mammalian expression plasmid, C-Myc tag||MG50173-CM|
|Mouse ACVR2B ORF mammalian expression plasmid, C-HA tag||MG50173-CY|
|Mouse ACVR2B Gene cDNA clone plasmid||MG50173-M|
|Mouse ACVR2B natural ORF mammalian expression plasmid||MG50173-M-N|
|Mouse ACVR2B ORF mammalian expression plasmid, N-Flag tag||MG50173-NF|
|Mouse ACVR2B ORF mammalian expression plasmid, N-His tag||MG50173-NH|
|Mouse ACVR2B ORF mammalian expression plasmid, N-Myc tag||MG50173-NM|
|Mouse ACVR2B ORF mammalian expression plasmid, N-HA tag||MG50173-NY|
|Mouse ACVR2B natural ORF mammalian expression plasmid||MG50173-UT|
|Learn more about expression Vectors|
ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.