|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse Axl Kinase ORF mammalian expression plasmid, C-GFPSpark tag||MG50126-ACG|
|Mouse Axl Kinase ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50126-ACR|
|Mouse Axl Kinase ORF mammalian expression plasmid, C-Flag tag||MG50126-CF|
|Mouse Axl Kinase ORF mammalian expression plasmid, C-His tag||MG50126-CH|
|Mouse Axl Kinase ORF mammalian expression plasmid, C-Myc tag||MG50126-CM|
|Mouse Axl Kinase ORF mammalian expression plasmid, C-HA tag||MG50126-CY|
|Mouse Axl Kinase Gene cDNA clone plasmid||MG50126-M|
|Mouse Axl Kinase ORF mammalian expression plasmid, N-Flag tag||MG50126-NF|
|Mouse Axl Kinase ORF mammalian expression plasmid, N-His tag||MG50126-NH|
|Mouse Axl Kinase ORF mammalian expression plasmid, N-Myc tag||MG50126-NM|
|Mouse Axl Kinase ORF mammalian expression plasmid, N-HA tag||MG50126-NY|
|Mouse Axl Kinase natural ORF mammalian expression plasmid||MG50126-UT|
|Learn more about expression Vectors|
Axl receptor tyrosine kinase, together with Tyro3 and Mer, constitute the TAM family of receptor tyrosine kinases. In the nervous system, Axl and its ligand Growth-arrest-specific protein 6 (Gas6) are expressed on multiple cell types. Axl functions in dampening the immune response, regulating cytokine secretion, clearing apoptotic cells and debris, and maintaining cell survival. Axl is upregulated in various disease states, such as in the cuprizone toxicity-induced model of demyelination and in multiple sclerosis (MS) lesions, suggesting that it plays a role in disease pathogenesis. Axl expression correlates with poor prognosis in several cancers. Axl mediates multiple oncogenic phenotypes and activation of these RTKs constitutes a mechanism of chemoresistance in a variety of solid tumors. Axl contributes to cell survival, migration, invasion, metastasis and chemosensitivity justify further investigation of Axl as novel therapeutic targets in cancer. The receptor tyrosine kinase AXL is thought to play a role in metastasis. The soluble AXL receptor as a therapeutic candidate agent for treatment of metastatic ovarian cancer. GAS6/AXL targeting as an effective strategy for inhibition of metastatic tumor progression in vivo.