|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SIGIRR ORF mammalian expression plasmid, C-GFPSpark tag||MG50122-ACG|
|Mouse SIGIRR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50122-ACR|
|Mouse SIGIRR ORF mammalian expression plasmid, C-Flag tag||MG50122-CF|
|Mouse SIGIRR ORF mammalian expression plasmid, C-His tag||MG50122-CH|
|Mouse SIGIRR ORF mammalian expression plasmid, C-Myc tag||MG50122-CM|
|Mouse SIGIRR ORF mammalian expression plasmid, C-HA tag||MG50122-CY|
|Mouse SIGIRR Gene cDNA clone plasmid||MG50122-M|
|Mouse SIGIRR ORF mammalian expression plasmid, N-Flag tag||MG50122-NF|
|Mouse SIGIRR ORF mammalian expression plasmid, N-His tag||MG50122-NH|
|Mouse SIGIRR ORF mammalian expression plasmid, N-Myc tag||MG50122-NM|
|Mouse SIGIRR ORF mammalian expression plasmid, N-HA tag||MG50122-NY|
|Mouse SIGIRR natural ORF mammalian expression plasmid||MG50122-UT|
|Learn more about expression Vectors|
Single Ig IL-1-related receptor (SIGIRR) or TIR8 is a member of Toll-like receptor-interleukin 1 receptor signaling (TLR-IL-1R) receptor superfamily. Although SIGIRR/TIR8 shows the typical conserved motifs that characterize the IL-1R and Toll superfamily, it is structurally and functionally distinct from both. SIGIRR/TIR8 has only one Ig domain in its extracellular portion whereas the IL-1R family contains three Ig folds. An unusually long cytoplasmic domain is reminiscent of the structure of drosophila Toll, yet the SIGIRR peptide sequence is more closely related to IL-1RI. SIGIRR/TIR8 was mainly expressed in mouse and human epithelial tissues such as kidney, lung and gut. Resting and activated T and B lymphocytes and monocytes-macrophages expressed little or no SIGIRR/TIR8, with the exception of the mouse GG2EE macrophage line. Inflammation is enhanced in SIGIRR-deficient mice. SIGIRR negatively modulates immune responses. Inflammation is enhanced in SIGIRR-deficient mice, as shown by their enhanced chemokine induction after IL-1 injection and reduced threshold for lethal endotoxin challenge.