|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human EPHA1 ORF mammalian expression plasmid, C-GFPSpark tag||HG15789-ACG|
|Human EPHA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG15789-ACR|
|Human EPHA1 ORF mammalian expression plasmid, C-Flag tag||HG15789-CF|
|Human EPHA1 ORF mammalian expression plasmid, C-His tag||HG15789-CH|
|Human EPHA1 ORF mammalian expression plasmid, C-Myc tag||HG15789-CM|
|Human EPHA1 ORF mammalian expression plasmid, C-HA tag||HG15789-CY|
|Human EPHA1 Gene cDNA clone plasmid||HG15789-G|
|Human EPHA1 ORF mammalian expression plasmid, N-Flag tag||HG15789-NF|
|Human EPHA1 ORF mammalian expression plasmid, N-His tag||HG15789-NH|
|Human EPHA1 ORF mammalian expression plasmid, N-Myc tag||HG15789-NM|
|Human EPHA1 ORF mammalian expression plasmid, N-HA tag||HG15789-NY|
|Human EPHA1 natural ORF mammalian expression plasmid||HG15789-UT|
|Learn more about expression Vectors|
EPHA1 or EPH receptor A1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. An important role of Eph receptors and their ligands ephrins is to mediate cell-contact-dependent repulsion. Eph receptors and ephrins also act at boundaries to channel neuronal growth cones along specific pathways, restrict the migration of neural crest cells, and via bidirectional signaling prevent intermingling between hindbrain segments. Eph receptors and ephrins can also trigger an adhesive response of endothelial cells and are required for the remodeling of blood vessels. Eph receptors and ephrins have emerged as key regulators of the repulsion and adhesion of cells that underlie the establishment, maintainence, and remodeling of patterns of cellular organization. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps.