|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse TGF-beta2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50153-ACG|
|Mouse TGF-beta2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50153-ACR|
|Mouse TGF-beta2 ORF mammalian expression plasmid, C-Flag tag||MG50153-CF|
|Mouse TGF-beta2 ORF mammalian expression plasmid, C-His tag||MG50153-CH|
|Mouse TGF-beta2 ORF mammalian expression plasmid, C-Myc tag||MG50153-CM|
|Mouse TGF-beta2 ORF mammalian expression plasmid, C-HA tag||MG50153-CY|
|Mouse TGF-beta2 Gene cDNA clone plasmid||MG50153-M|
|Mouse TGF-beta2 ORF mammalian expression plasmid, N-Flag tag||MG50153-NF|
|Mouse TGF-beta2 ORF mammalian expression plasmid, N-His tag||MG50153-NH|
|Mouse TGF-beta2 ORF mammalian expression plasmid, N-Myc tag||MG50153-NM|
|Mouse TGF-beta2 ORF mammalian expression plasmid, N-HA tag||MG50153-NY|
|Mouse TGF-beta2 natural ORF mammalian expression plasmid||MG50153-UT|
|Learn more about expression Vectors|
TGF beta 2 (Transforming growth factor beta 2), an extracellular glycosylated protein, which belongs to the TGF-beta family. TGF-beta regulates key mechanisms of tumor development, namely immunosuppression, metastasis, angiogenesis, and proliferation. TGF beta 2 suppression is a promising therapeutic approach for malignant tumor therapy. The signaling pathway of TGF beta 2/Smad plays an important role in the pathological process in posterior capsule opacification (PCO) after cataract surgery. Silencing Smad2 and Smad3 efficiently blocked the effect of TGF beta 2 on cell proliferation, migration, and extracellular matrix production. TGF beta 2 activation of MEKK3/ERK1/2/5 signaling modulates Has2 expression and hyaluronan (HA) production leading to the induction of epithelial to mesenchymal transformation (EMT) events. In addition, the upregulation of the TGF beta 2 level is a common pathological feature of Alzheimer's disease (AD) brains and suggests that it may be closely linked to the development of neuronal death related to AD.