|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse RTN4R / NOGOR ORF mammalian expression plasmid, C-GFPSpark tag||MG50106-ACG|
|Mouse RTN4R / NOGOR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50106-ACR|
|Mouse RTN4R / NOGOR ORF mammalian expression plasmid, C-Flag tag||MG50106-CF|
|Mouse RTN4R / NOGOR ORF mammalian expression plasmid, C-His tag||MG50106-CH|
|Mouse RTN4R / NOGOR ORF mammalian expression plasmid, C-Myc tag||MG50106-CM|
|Mouse RTN4R / NOGOR ORF mammalian expression plasmid, C-HA tag||MG50106-CY|
|Mouse RTN4R / NOGOR Gene cDNA clone plasmid||MG50106-M|
|Mouse RTN4R / NOGOR ORF mammalian expression plasmid, N-Flag tag||MG50106-NF|
|Mouse RTN4R / NOGOR ORF mammalian expression plasmid, N-His tag||MG50106-NH|
|Mouse RTN4R / NOGOR ORF mammalian expression plasmid, N-Myc tag||MG50106-NM|
|Mouse RTN4R / NOGOR ORF mammalian expression plasmid, N-HA tag||MG50106-NY|
|Mouse RTN4R / NOGOR natural ORF mammalian expression plasmid||MG50106-UT|
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Reticulon 4 receptor (RTN4R), also known as Nogo-66 Receptor (NgR), is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the Nogo recptor family including three members. Mouse RTN4R cDNA contains 10 LRP (Leucine-rich) repeats. RTN4R is expressed predominantly in neurons and their axons in the central nervous systems (CNS). As a receptor for myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMG), RTN4R mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult CNS. It has been shown that RTN4R performs its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a TNFRSF member also known for modulating the activities of the Trk family and for inducing apoptosis in neurons and oligodendrocytes. RTN4R may be proposed as a potential drug target for treatment of various neurological conditions such as spinal cord injury, CNS lesions, peripheral nerve injury, stroke and Alzheimer's disease (AD). Additionally, RTN4R may play a role in regulating the function of the gap junctions.
1. Wang, X. et al., 2006, Ann Neurol. 60(5): 540-549.
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3. Zhu, H.Y. et al., 2007, Hum Pathol. 38(3): 426-434.
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