|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CCL24 ORF mammalian expression plasmid, C-GFPSpark tag||MG50104-ACG|
|Mouse CCL24 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50104-ACR|
|Mouse CCL24 ORF mammalian expression plasmid, C-Flag tag||MG50104-CF|
|Mouse CCL24 ORF mammalian expression plasmid, C-His tag||MG50104-CH|
|Mouse CCL24 ORF mammalian expression plasmid, C-Myc tag||MG50104-CM|
|Mouse CCL24 ORF mammalian expression plasmid, C-HA tag||MG50104-CY|
|Mouse CCL24 Gene cDNA clone plasmid||MG50104-M|
|Mouse CCL24 ORF mammalian expression plasmid, N-Flag tag||MG50104-NF|
|Mouse CCL24 ORF mammalian expression plasmid, N-His tag||MG50104-NH|
|Mouse CCL24 ORF mammalian expression plasmid, N-Myc tag||MG50104-NM|
|Mouse CCL24 ORF mammalian expression plasmid, N-HA tag||MG50104-NY|
|Mouse CCL24 natural ORF mammalian expression plasmid||MG50104-UT|
|Learn more about expression Vectors|
CCL24, also known as Eotaxin-2 and MPIF-2, belongs to the intercrine beta (chemokine CC) family. CCL24 gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. CCL24 displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. CCL24 is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. CCL24 is chemotactic for resting T-lymphocytes, and eosinophils. It has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. It is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Eotaxin-2 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils. Elevated level of Eotaxin-2 has been seen in patients with aspirin-exacerbated respiratory disease.