|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CD99L2 ORF mammalian expression plasmid, C-GFPSpark tag||HG15760-ACG|
|Human CD99L2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG15760-ACR|
|Human CD99L2 ORF mammalian expression plasmid, C-Flag tag||HG15760-CF|
|Human CD99L2 ORF mammalian expression plasmid, C-His tag||HG15760-CH|
|Human CD99L2 ORF mammalian expression plasmid, C-Myc tag||HG15760-CM|
|Human CD99L2 ORF mammalian expression plasmid, C-HA tag||HG15760-CY|
|Human CD99L2 Gene cDNA clone plasmid||HG15760-G|
|Human CD99L2 ORF mammalian expression plasmid, N-Flag tag||HG15760-NF|
|Human CD99L2 ORF mammalian expression plasmid, N-His tag||HG15760-NH|
|Human CD99L2 ORF mammalian expression plasmid, N-Myc tag||HG15760-NM|
|Human CD99L2 ORF mammalian expression plasmid, N-HA tag||HG15760-NY|
|Human CD99L2 natural ORF mammalian expression plasmid||HG15760-UT|
|Learn more about expression Vectors|
Mouse CD99 antigen-like protein 2, also known as MIC2-like protein 1, CD99L2 and MIC2L1, is a single-pass type I membrane protein which belongs to the CD99 family. CD99L2 is expressed in brain, heart, lung, liver, spleen, kidney, stomach, small intestine, skeletal muscle, ovary, thymus, testis and uterus. Lower expression of CD99L2 is seen in thymus. It is also expressed in E18 uterus and placenta. CD99 and CD99L2 were required for leukocyte extravasation in the cremaster after stimulation with tumor necrosis factor-alpha, where the need for PECAM-1 is known to be bypassed. CD99 and CD99L2 act independently of PECAM-1 in leukocyte extravasation and cooperate in an independent way to help neutrophils overcome the endothelial basement membrane. CD99L2 may function as a homophilic adhesion molecule. It functions in leukocyte-endothelial cell interactions during leukocyte extravasation, and in particular, at the diapedesis step. CD99L2 does not seem to be involved in docking of leukocytes to the vessel wall or in lymphocyte diapedesis.