|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse TFPI ORF mammalian expression plasmid, C-GFPSpark tag||MG50131-ACG|
|Mouse TFPI ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50131-ACR|
|Mouse TFPI ORF mammalian expression plasmid, C-Flag tag||MG50131-CF|
|Mouse TFPI ORF mammalian expression plasmid, C-His tag||MG50131-CH|
|Mouse TFPI ORF mammalian expression plasmid, C-Myc tag||MG50131-CM|
|Mouse TFPI ORF mammalian expression plasmid, C-HA tag||MG50131-CY|
|Mouse TFPI Gene cDNA clone plasmid||MG50131-M|
|Mouse TFPI ORF mammalian expression plasmid, N-Flag tag||MG50131-NF|
|Mouse TFPI ORF mammalian expression plasmid, N-His tag||MG50131-NH|
|Mouse TFPI ORF mammalian expression plasmid, N-Myc tag||MG50131-NM|
|Mouse TFPI ORF mammalian expression plasmid, N-HA tag||MG50131-NY|
|Mouse TFPI natural ORF mammalian expression plasmid||MG50131-UT|
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Tissue factor pathway inhibitor (TFPI) is the natural inhibitor of TF coagulant and signaling activities. It is a Kunitz-type serine proteinase inhibitor that down-regulates tissue factor-initiated blood coagulation. With its Kunitz domains, TFPI exhibits significant homology with human inter-alpha-trypson inhibitor and bovin basic pancreatic trypsin inhibitor. TFPI is the natural inhibitor of TF coagulant and signaling activities. The importance of TFPI in the regulation of blood coagulation is emphasized by how its activity is modulated in human disease. In a factor (F) Xa-dependent feedback system, TFPI binds directly and inhibits the TF-FVII/FVIIa complex. Normally, TFPI exists in plasma both as a full-length molecule and as variably carboxy-terminal truncated forms. TFPI also circulates in complex with plasma lipoproteins. The levels and the dual inhibitor effect of TFPI on FXa and TF-FVII/FVIIa complex offers insight into the mechanisms of various pathological conditions triggered by TF. TFPI may play an important role in modulating TF-induced thrombogenesis and it may also provide a unique therapeutic approach for prophylaxis and/or treatment of various diseases. In addition, Studies have shown that TFPI exhibits antiangiogenic and antimetastatic effects in vitro and in vivo. In animal models of experimental metastasis, both circulating and tumor cell-associated TFPI are shown to significantly reduce tumor cell-induced coagulation activation and lung metastasis.