|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse NRN1 ORF mammalian expression plasmid, C-GFPSpark tag||MG52152-ACG|
|Mouse NRN1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52152-ACR|
|Mouse NRN1 ORF mammalian expression plasmid, C-Flag tag||MG52152-CF|
|Mouse NRN1 ORF mammalian expression plasmid, C-His tag||MG52152-CH|
|Mouse NRN1 ORF mammalian expression plasmid, C-Myc tag||MG52152-CM|
|Mouse NRN1 ORF mammalian expression plasmid, C-HA tag||MG52152-CY|
|Mouse NRN1 Gene cDNA clone plasmid||MG52152-G|
|Mouse NRN1 ORF mammalian expression plasmid, N-Flag tag||MG52152-NF|
|Mouse NRN1 ORF mammalian expression plasmid, N-His tag||MG52152-NH|
|Mouse NRN1 ORF mammalian expression plasmid, N-Myc tag||MG52152-NM|
|Mouse NRN1 ORF mammalian expression plasmid, N-HA tag||MG52152-NY|
|Mouse NRN1 natural ORF mammalian expression plasmid||MG52152-UT|
|Learn more about expression Vectors|
Neuritin 1 (NRN1) is a member of neuritin family. Neuritin is a glycosylphosphatidylinositol- anchored protein induced by neural activity. It is expressed in postmitotic-differentiating neurons of the developing nervous system and a population of small-diameter neurons in the dorsal root ganglia and was anterogradely and retrogradely transported. Neuritin message is induced by neuronal activity and by the activity-regulated neurotrophins BDNF, nerve growth factor (NGF) and NT-3. Purified recombinant neuritin promotes neurite outgrowth and arborization in primary embryonic hippocampal and cortical cultures. Thus, neuritin is considered as a downstream effector of activity-induced neurite outgrowth. In clinical, neuritin levels in diabetes were reduced in both dorsal root ganglia and sciatic nerve of rats, and these deficits were reversed in vivo by treatment with NGF. This manipulation of neuritin levels in diabetes may provide a potential target for the therapeutic intervention in the management of neuropathy.