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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Mouse DCN ORF mammalian expression plasmid, C-GFPSpark tag||MG51501-ACG|
|Mouse DCN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51501-ACR|
|Mouse DCN ORF mammalian expression plasmid, C-Flag tag||MG51501-CF|
|Mouse DCN ORF mammalian expression plasmid, C-His tag||MG51501-CH|
|Mouse DCN ORF mammalian expression plasmid, C-Myc tag||MG51501-CM|
|Mouse DCN ORF mammalian expression plasmid, C-HA tag||MG51501-CY|
|Mouse DCN ORF mammalian expression plasmid, N-Flag tag||MG51501-NF|
|Mouse DCN ORF mammalian expression plasmid, N-His tag||MG51501-NH|
|Mouse DCN ORF mammalian expression plasmid, N-Myc tag||MG51501-NM|
|Mouse DCN ORF mammalian expression plasmid, N-HA tag||MG51501-NY|
|Mouse DCN Gene cDNA clone plasmid||MG51501-U|
|Mouse DCN natural ORF mammalian expression plasmid||MG51501-UT|
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Decorin is a ubiquitous small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a core protein and a covalently linked glycosaminoglycan chain which is either chondroitin sulfate (CS) or dermatan sulfate (DS). As a component of connective tissue, decorin interacts with several extracellular matrix components, such as type I collagen and fibronectin, and plays a role in matrix assembly. Decorin resides in the tumor microenvironment and affects the biology of various types of cancer by downregulating the activity of several receptors involved in cell growth and survival. Decorin binds to and modulates the signaling of the epidermal growth factor receptor and other members of the ErbB family of receptor tyrosine kinases. It exerts its antitumor activity by a dual mechanism: via inhibition of these key receptors through their physical downregulation coupled with attenuation of their signaling, and by binding to and sequestering TGFbeta. Decorin also modulates the insulin-like growth factor receptor and the low-density lipoprotein receptor-related protein 1, which indirectly affects the TGFbeta receptor pathway. Decorin plays significant roles in tissue development and assembly, as well as playing both direct and indirect signaling roles.