|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SERPIND1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50121-ACG|
|Mouse SERPIND1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50121-ACR|
|Mouse SERPIND1 ORF mammalian expression plasmid, C-Flag tag||MG50121-CF|
|Mouse SERPIND1 ORF mammalian expression plasmid, C-His tag||MG50121-CH|
|Mouse SERPIND1 ORF mammalian expression plasmid, C-Myc tag||MG50121-CM|
|Mouse SERPIND1 ORF mammalian expression plasmid, C-HA tag||MG50121-CY|
|Mouse SERPIND1 Gene cDNA clone plasmid||MG50121-M|
|Mouse SERPIND1 ORF mammalian expression plasmid, N-Flag tag||MG50121-NF|
|Mouse SERPIND1 ORF mammalian expression plasmid, N-His tag||MG50121-NH|
|Mouse SERPIND1 ORF mammalian expression plasmid, N-Myc tag||MG50121-NM|
|Mouse SERPIND1 ORF mammalian expression plasmid, N-HA tag||MG50121-NY|
|Mouse SERPIND1 natural ORF mammalian expression plasmid||MG50121-UT|
|Learn more about expression Vectors|
SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.