|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse Pglyrp1 / PGRP ORF mammalian expression plasmid, C-GFPSpark tag||MG50115-ACG|
|Mouse Pglyrp1 / PGRP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50115-ACR|
|Mouse Pglyrp1 / PGRP ORF mammalian expression plasmid, C-Flag tag||MG50115-CF|
|Mouse Pglyrp1 / PGRP ORF mammalian expression plasmid, C-His tag||MG50115-CH|
|Mouse Pglyrp1 / PGRP ORF mammalian expression plasmid, C-Myc tag||MG50115-CM|
|Mouse Pglyrp1 / PGRP ORF mammalian expression plasmid, C-HA tag||MG50115-CY|
|Mouse Pglyrp1 / PGRP Gene cDNA clone plasmid||MG50115-M|
|Mouse Pglyrp1 / PGRP ORF mammalian expression plasmid, N-Flag tag||MG50115-NF|
|Mouse Pglyrp1 / PGRP ORF mammalian expression plasmid, N-His tag||MG50115-NH|
|Mouse Pglyrp1 / PGRP ORF mammalian expression plasmid, N-Myc tag||MG50115-NM|
|Mouse Pglyrp1 / PGRP ORF mammalian expression plasmid, N-HA tag||MG50115-NY|
|Mouse Pglyrp1 / PGRP natural ORF mammalian expression plasmid||MG50115-UT|
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Mouse Peptidoglycan recognition protein 1, also known as Peptidoglycan recognition protein short, PGRP-S, PGLYRP1, PGLYRP, PGRP and TNFSF3L, is a secreted protein which belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. PGLYRP1 / PGLYRP is highly expressed in bone marrow. It is weakly expressed in kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, lung, fetal spleen and neutrophils. PGLYRP1 / PGLYRP is a pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. It has bactericidal activity towards Gram-positive bacteria. PGLYRP1 / PGLYRP may kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. It binds also to Gram-negative bacteria, and has bacteriostatic activity towards Gram-negative bacteria.
Peptidoglycan recognition proteins ( PGRPs or PGLYRPs ) are innate immunity proteins that are conserved from insects to mammals, recognize bacterial peptidoglycan, and function in antibacterial immunity and inflammation. Mammals have four PGRPs: PGLYRP1, PGLYRP2, PGLYRP3, and PGLYRP4. They are secreted proteins expressed in polymorphonuclear leukocytes ( PGLYRP1 ), liver ( PGLYRP2 ), or on body surfaces, mucous membranes, and in secretions (saliva, sweat) (PGLYRP3 and PGLYRP4). All PGRPs recognize bacterial peptidoglycan. The PGRPs likely play a role both in antibacterial defenses and several inflammatory diseases. They modulate local inflammatory responses in tissues (such as arthritic joints) and there is evidence for association of PGRPs with inflammatory diseases, such as psoriasis.